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Ce(IV)-catalyzed site-selective DNA hydrolysis using tris- and tetrakismethylenephosphonate-modified oligonucleotides.

作者信息

Lönnberg Tuomas, Suzuki Yuta, Komiyama Makoto

机构信息

RCAST, The University of Tokyo, 4-6-1, Komaba, Meguro-Ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):195-6. doi: 10.1093/nass/nrm098.

DOI:10.1093/nass/nrm098
PMID:18029653
Abstract

To improve the recruitment of the catalytically active Ce(IV) ion to the DNA cleavage site in the ARCUT (Artificial Restriction DNA Cutter) system, two phosphonate ligands, nitrilotris(methylenephosphonic acid) (NTP) and N,N,N',N'-ethylenediaminetetra-(methylenephosphonic acid) (EDTP), have been linked to the 5'- or 3'-end of oligonucleotides complementary to the target DNA. Compared to their counterparts bearing a terminal monophosphate, these new modified oligonucleotides exhibit a modest to significant increase in the cleavage activity, depending on the target.

摘要

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