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沙门氏菌S:41:z10乳糖特性的研究

Studies on the lactose character in Salmonella S:41:z10.

作者信息

Rajgopalan D, Sathe M J, Joshi L, Deshpande C K

机构信息

Department of Molecular Biology, Seth G. S. Medical College, Parel, Bombay, Maharashtra.

出版信息

J Postgrad Med. 1991 Apr;37(2):93-6.

PMID:1803005
Abstract

A number of plasmids carrying the Lac+ character have been reported. Lac+ character of salmonella S:41:z10:- studied for transfer of Lac+ character to standard Escherichia coli K12 Lac-F- Nalr and Escherichia coli K12 F- Lac- Rifr, failed to transfer in in vitro experiments. Similarly, identification and characterisation of plasmid DNA by agarose gel electrophoresis technique did not show specific plasmid DNA as compared to standard molecular weight plasmids. Plasmid DNA appeared to have been embedded with chromosomal DNA molecule.

摘要

已经报道了许多携带Lac+性状的质粒。对沙门氏菌S:41:z10:-的Lac+性状进行研究,以将Lac+性状转移到标准大肠杆菌K12 Lac-F- Nalr和大肠杆菌K12 F- Lac- Rifr中,但在体外实验中未能成功转移。同样,与标准分子量质粒相比,通过琼脂糖凝胶电泳技术对质粒DNA进行鉴定和表征时,未显示出特定的质粒DNA。质粒DNA似乎已与染色体DNA分子嵌入在一起。

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