Corcoran K D, Thoen C O
United States Army Medical Research Institute of Chemical Defense, Veterinary Medicine and Laboratory Resources Division, Aberdeen Proving Ground, MD 21010-5425.
J Med Primatol. 1991 Oct;20(8):404-8.
An enzyme immunoassay (EIA) was developed for detecting mycobacterial antibodies in the sera of 22 Macaca fascicularis following a natural outbreak of tuberculosis. EIAs were conducted using four antigens (lysozyme, triton, or deoxycholate extracts of Mycobacterium tuberculosis or a purified protein derivative) and two conjugates (protein A or antihuman). Mycobacterial antibodies were detected in two of two culture-positive monkeys, in nine of ten tuberculin test-suspect monkeys (culture-negative), and in five of ten tuberculin test-negative monkeys (culture-negative). Results indicate EIA may be of practical value in detecting monkeys exposed to M. tuberculosis.
为检测22只食蟹猴在自然爆发结核病后的血清中分枝杆菌抗体，开发了一种酶免疫测定法（EIA）。使用四种抗原（结核分枝杆菌的溶菌酶、曲拉通或脱氧胆酸盐提取物或纯化蛋白衍生物）和两种结合物（蛋白A或抗人）进行酶免疫测定。在两只培养阳性的猴子中检测到了分枝杆菌抗体，在十只结核菌素试验疑似猴子（培养阴性）中的九只中检测到了分枝杆菌抗体，在十只结核菌素试验阴性猴子（培养阴性）中的五只中检测到了分枝杆菌抗体。结果表明，酶免疫测定法在检测接触结核分枝杆菌的猴子方面可能具有实际价值。
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