Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P. R. China.
Exp Anim. 2013;62(4):281-93. doi: 10.1538/expanim.62.281.
Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. In this study, we first sought to establish an appropriate primate TB model resembling natural TB in nonhuman primates. Four rhesus monkeys (Macaca mulatta) of Chinese origin were infected intratracheally with two low doses of M. tuberculosis H37Rv. Regardless of the infectious doses, all monkeys were demonstrated to be successfully infected by clinical assessments, tuberculin skin test conversions, peripheral immune responses, gross observations, histopathology analysis, and M. tuberculosis burdens. Furthermore, we extended the usefulness of this model for assessing the following immunodiagnostic antigens: CFP10, ESAT-6, CFP10-ESAT-6, and an antigen cocktail of CFP10 and ESAT-6. The data showed that CFP10 was an M. tuberculosis-specific, "early" antigen used for serodiagnosis of TB in nonhuman primates. In conclusion, we established a useful primate TB model depending on low doses of M .tuberculosis and affording new opportunities for studies of M. tuberculosis disease and diagnostics.
非人类灵长类动物中的结核病（TB）对与它们接触的动物和人类的福利构成了严重威胁，而快速、准确、稳健的诊断具有挑战性。在这项研究中，我们首先试图建立一种适当的灵长类 TB 模型，该模型类似于非人类灵长类动物中的自然 TB。我们对 4 只来自中国的恒河猴（Macaca mulatta）进行了气管内感染，用两个低剂量的结核分枝杆菌 H37Rv 进行感染。无论感染剂量如何，所有猴子均通过临床评估、结核菌素皮肤试验转换、外周免疫反应、大体观察、组织病理学分析和结核分枝杆菌负担证实成功感染。此外，我们扩展了该模型在评估以下免疫诊断抗原方面的用途：CFP10、ESAT-6、CFP10-ESAT-6 和 CFP10 和 ESAT-6 的抗原混合物。数据表明，CFP10 是一种结核分枝杆菌特异性的“早期”抗原，可用于灵长类动物结核病的血清学诊断。总之，我们建立了一种基于低剂量结核分枝杆菌的有用的灵长类 TB 模型，为结核分枝杆菌疾病和诊断研究提供了新的机会。
