Schulze Philipp, Schüttpelz Mark, Sauer Markus, Belder Detlev
Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103, Leipzig, Germany.
Lab Chip. 2007 Dec;7(12):1841-4. doi: 10.1039/b710762e. Epub 2007 Oct 10.
Two photon excited (TPE) fluorescence detection was applied to native fluorescence detection of aromatics in microchip electrophoresis (MCE). This technique was evaluated as an alternative to common one photon excitation in the deep UV spectral range. TPE enables fluorescence detection of unlabeled aromatic compounds, even in non-deep UV-transparent microfluidic chips. In this study, we demonstrate the proof of concept of native TPE fluorescence detection of small aromatics in commercial microfluidic glass chips. Label-free TPE fluorescence detection of native proteins and small aromatics in MCE was achieved within the micromolar concentration range, utilising 420 nm excitation light.
双光子激发（TPE）荧光检测被应用于微芯片电泳（MCE）中芳烃的天然荧光检测。该技术被评估为深紫外光谱范围内常见单光子激发的替代方法。TPE能够对未标记的芳香族化合物进行荧光检测，即使在非深紫外透明的微流控芯片中也能实现。在本研究中，我们展示了在商用微流控玻璃芯片中对小分子芳烃进行天然TPE荧光检测的概念验证。利用420 nm激发光，在微摩尔浓度范围内实现了MCE中天然蛋白质和小分子芳烃的无标记TPE荧光检测。
