Abrat O B, Semchyshyn H M, Lushchak V I
Ukr Biokhim Zh (1999). 2007 Mar-Apr;79(2):17-23.
The effect of acetic acid on survival and activity of superoxide dismutase and catalase in different Saccharomyces cerevisiae strains has been studied. The cell survival decreases with an increase of acetic acid concentration in all strains studied. YBB14 (deltapdr12) and YAK120 (deltawar1) strains are more sensitive to acetate than their parental strain W303-1A. It can be assumed that ABC-transporter Pdr12p and its transcriptional regulator War1p play an important role in the transportation of acetic acid from the cell. Incubation of the yeast with 150-200 mM CH3COOH causes the increase of superoxide dismutase and catalase activities in the wild strain. At the same time, the enzyme activities rise with the increase of time exposure. However, superoxide dismutase and catalase deficit does not decrease yeast survival under conditions used in this study.
研究了乙酸对不同酿酒酵母菌株中超氧化物歧化酶和过氧化氢酶存活及活性的影响。在所研究的所有菌株中，细胞存活率随乙酸浓度的增加而降低。YBB14（deltapdr12）和YAK120（deltawar1）菌株比其亲本菌株W303 - 1A对乙酸更敏感。可以推测ABC转运蛋白Pdr12p及其转录调节因子War1p在乙酸从细胞的转运中起重要作用。用150 - 200 mM CH3COOH孵育酵母会导致野生菌株中超氧化物歧化酶和过氧化氢酶活性增加。同时，酶活性随暴露时间的增加而升高。然而，在本研究使用的条件下，超氧化物歧化酶和过氧化氢酶缺陷并未降低酵母存活率。
