Markov A G, Veshniakova A Iu, Krug S, Milatz S
Ross Fiziol Zh Im I M Sechenova. 2007 Sep;93(9):1043-54.
Proteins' expression of tight junction proteins in rat small intestine was analyzed by means of immunohistochemistry and Western blotting. Claudins -1, -2, -3, -4, -5, -7 and occludin were analyzed. Confocal laser-scanning microscopy revealed differences in the subcellular distributions of these proteins. Claudins-1, -2, -3, -4, -5, and -7 were localized within the tight junction complex and were also detectable in subjunctional regions. Distributed subcellular localization of these proteins may reflect their different roles during formation of epithelial cell layers as well as in paracellular transport of ions in the small intestine.
采用免疫组织化学和蛋白质印迹法分析大鼠小肠紧密连接蛋白的表达。分析了闭合蛋白-1、-2、-3、-4、-5、-7和闭合蛋白。共聚焦激光扫描显微镜揭示了这些蛋白质在亚细胞分布上的差异。闭合蛋白-1、-2、-3、-4、-5和-7定位于紧密连接复合体中，在连接下区域也可检测到。这些蛋白质的亚细胞分布差异可能反映了它们在小肠上皮细胞层形成过程中以及离子细胞旁转运中的不同作用。
