Pacheco Neith, Larralde-Corona C Patricia, Sepulveda Jose, Trombotto Stéphan, Domard Alain, Shirai Keiko
Universidad Autónoma Metropolitana, Departamento de Biotecnología, Laboratorio de Biopolímeros, Mexico City, Mexico.
Int J Biol Macromol. 2008 Jul 1;43(1):20-6. doi: 10.1016/j.ijbiomac.2007.10.009. Epub 2007 Oct 18.
Chitosans were obtained by room-temperature-homogeneous-deacetylation (RTHD) and freeze-pump-out-thaw-heterogeneous-deacetylation (FPT) from chitins purified from fermentations. Commercial chitosan was deacetylated by three-FPT-cycles. Chitosans and Pichia guillermondii were evaluated on the growth of Penicillium digitatum. Medium molecular weight (M(W)) chitosans displayed higher inhibitory activity against the yeast than low M(W) biopolymers. Chitosans with low degree of acetylation (DA) were inhibitory for yeast and mould. Therefore, a low M(W) and high DA chitosan was selected for use against moulds combined with yeasts. Biopolymer and yeasts presented an additive effect, since chitosans were effective to delay spore germination, whereas yeast decreased apical fungal growth.
壳聚糖通过室温均匀脱乙酰化（RTHD）和冷冻-抽气-解冻非均匀脱乙酰化（FPT）从发酵纯化的几丁质中获得。商业壳聚糖通过三个FPT循环进行脱乙酰化。对壳聚糖和季也蒙毕赤酵母对指状青霉生长的影响进行了评估。中等分子量（M（W））的壳聚糖比低M（W）生物聚合物对酵母表现出更高的抑制活性。低乙酰化度（DA）的壳聚糖对酵母和霉菌具有抑制作用。因此，选择了低M（W）和高DA的壳聚糖用于对抗霉菌和酵母。生物聚合物和酵母呈现出相加效应，因为壳聚糖可有效延迟孢子萌发，而酵母则可降低真菌顶端生长。
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