Departamento de Processos Químicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas , Campinas, SP , Brasil.
Braz J Microbiol. 2012 Apr;43(2):800-9. doi: 10.1590/S1517-83822012000200046. Epub 2012 Jun 1.
The inhibitory effects of fifteen chitosans with different degrees of polymerization (DP) and different degrees of acetylation (FA) on the growth rates (GR) of four phytopathogenic fungi (Alternaria alternata, Botrytis cinerea, Penicillium expansum, and Rhizopus stolonifer) were examined using a 96-well microtiter plate and a microplate reader. The minimum inhibitory concentrations (MICs) of the chitosans ranged from 100 μg ×mL(-1) to 1,000 μg ×mL(-1) depending on the fungus tested and the DP and FA of the chitosan. The antifungal activity of the chitosans increased with decreasing FA. Chitosans with low FA and high DP showed the highest inhibitory activity against all four fungi. P. expansum and B. cinerea were relatively less susceptible while A. alternata and R. stolonifer were relatively more sensitive to the chitosan polymers. Scanning electron microscopy of fungi grown on culture media amended with chitosan revealed morphological changes.
采用 96 孔微量滴定板和微量板读数仪，研究了 15 种聚合度（DP）和乙酰化度（FA）不同的壳聚糖对 4 种植物病原菌（链格孢菌、灰葡萄孢菌、扩展青霉和腐生枝孢菌）生长率（GR）的抑制作用。壳聚糖的最低抑菌浓度（MIC）范围为 100 μg×mL(-1)至 1,000 μg×mL(-1)，具体取决于所测试的真菌以及壳聚糖的 DP 和 FA。壳聚糖的抗真菌活性随 FA 的降低而增加。低 FA 和高 DP 的壳聚糖对所有 4 种真菌表现出最高的抑制活性。扩展青霉和灰葡萄孢菌相对较不敏感，而链格孢菌和腐生枝孢菌相对较敏感于壳聚糖聚合物。在添加壳聚糖的培养基上培养真菌的扫描电子显微镜显示出形态变化。
