Tucker W D, Noble W C
Department of Microbiology, Central Middlesex Hospital, Park Royal, London, UK.
Mycoses. 1991 Jul-Aug;34(7-8):303-7. doi: 10.1111/j.1439-0507.1991.tb00664.x.
The paper reports observations made of various Microsporum species during a PAGE pattern study of the epidemiology of Microsporum canis. It is shown that M. audouinii can readily be distinguished from M. canis but that M. equinum is more difficult to separate. M. rivalierii was distinguishable from M. audouinii, confirming previous reports. M. distortum could not be separated from M. canis by PAGE gels of total protein or by isozyme patterns generated. There are particular problems with studies of that type on dermatophyte protein in that the intrinsic proteases degrade the preparations.
该论文报告了在犬小孢子菌流行病学的聚丙烯酰胺凝胶电泳(PAGE)图谱研究中对各种小孢子菌属菌种的观察结果。结果表明,奥杜盎小孢子菌很容易与犬小孢子菌区分开来,但马小孢子菌则更难分离。里瓦利耶小孢子菌可与奥杜盎小孢子菌区分开,这证实了先前的报道。通过总蛋白的PAGE凝胶或所产生的同工酶图谱,变形小孢子菌无法与犬小孢子菌区分开来。对皮肤癣菌蛋白质进行此类研究存在特殊问题,因为内在蛋白酶会降解制剂。