Tucker W D, Noble W C
Public Health Laboratory, Central Middlesex Hospital, London, UK.
J Med Vet Mycol. 1990;28(2):117-23. doi: 10.1080/02681219080000161.
Whole-cell protein analysis of clinical isolates of Microsporum canis by polyacrylamide gel electrophoresis (PAGE) showed small but inconsistent differences in protein patterns. All clinical isolates closely resembled the (-) mating type of Arthroderma otae. A reference strain of A. otae (+) mating type from Japan gave a PAGE pattern which was distinct from those of the clinical isolates. Mating studies showed that the progeny of these reference (+) and (-) mating types had a variety of distinct protein patterns. Protein patterns can therefore distinguish between genetically different strains of M. canis (A. otae) suggesting that clinical isolates have a common clonal origin.
通过聚丙烯酰胺凝胶电泳（PAGE）对犬小孢子菌临床分离株进行全细胞蛋白质分析，结果显示蛋白质图谱存在微小但不一致的差异。所有临床分离株与奥塔节皮菌的（-）交配型极为相似。一株来自日本的奥塔节皮菌（+）交配型参考菌株给出的PAGE图谱与临床分离株的图谱不同。交配研究表明，这些参考（+）和（-）交配型的后代具有多种不同的蛋白质图谱。因此，蛋白质图谱可以区分犬小孢子菌（奥塔节皮菌）的不同遗传菌株，这表明临床分离株有共同的克隆起源。
