Thomsen M K, Friis C, Nielsen P
Department of Pharmacology, Leo Pharmaceutical Products, Ballerup, Denmark.
Pharmacol Toxicol. 1991 Nov;69(5):381-5. doi: 10.1111/j.1600-0773.1991.tb01316.x.
Different cytochrome P-450 isoenzymes from hepatic microsomes of phenobarbital (PB) and beta-naphthoflavone (beta-NF) treated pigs and rats were isolated, purified, and characterized. The physico-chemical properties of the porcine isoenzymes were similar to properties of forms isolated from other species. The molecular sizes ranged from 52.5 to 59.5 kD and, in the ferrous-carbonyl state, the isoenzymes had absorbance maxima between 447 and 451 nm. Antigenic similarities were found between the isoenzymes present in PB-induced pigs, and between the isoenzymes present in beta-NF-induced pigs. Cross-reactivity was not observed between PB- and beta-NF-inducible isoenzymes, but beta-NF-inducible isoenzymes in pigs and rats possessed antigenic similarities.
从经苯巴比妥（PB）和β-萘黄酮（β-NF）处理的猪和大鼠的肝微粒体中分离、纯化并鉴定了不同的细胞色素P-450同工酶。猪同工酶的物理化学性质与从其他物种分离出的形式的性质相似。分子大小在52.5至59.5kD之间，在亚铁羰基状态下，同工酶的最大吸光度在447至451nm之间。在PB诱导的猪中存在的同工酶之间，以及在β-NF诱导的猪中存在的同工酶之间发现了抗原相似性。在PB诱导型和β-NF诱导型同工酶之间未观察到交叉反应，但猪和大鼠中的β-NF诱导型同工酶具有抗原相似性。
