Sun J Q, Lau P P, Strobel H W
Exp Gerontol. 1986;21(2):65-73. doi: 10.1016/0531-5565(86)90054-9.
The various forms of hepatic cytochrome P-450 respond differentially to aging and induction. We examined the levels of six forms of cytochrome P-450, designated as Forms 1 through 5 and Form b, as a function of age and induction. Radial immunodiffusion analysis of rat liver microsomes indicate that cytochrome P-450 Forms 1 and 2 respond to induction by beta-naphthoflavone or phenobarbital less well in aging rats than in young rats. beta-naphthoflavone is less effective in inducing Forms 3, 4, and 5 in aging rats than in young rats. Phenobarbital, however, is more effective in inducing Forms 3 and 4 in aging rats than in young rats but does not induce Form 5 in either young or aging rats. Although Form b is induced predominantly by phenobarbital, beta-naphthoflavone induces Form b moderately in aging rats. Phenobarbital induces Form b to approximately the same extent in aging rats and in young rats. In untreated rats Form 2 is the predominant form, while Forms 1 and 3 are present in moderate amounts. The results of the immunodiffusion analysis were confirmed by the resolution and partial purification of cytochromes P-450 from microsomes of aging and young rats pretreated with beta-naphthoflavone or phenobarbital. These results identify changes with age in specific forms of cytochrome P-450 as a function of the aging process in rats.
肝脏细胞色素P - 450的各种形式对衰老和诱导的反应各不相同。我们研究了六种细胞色素P - 450形式（分别命名为1至5型和b型）的水平随年龄和诱导作用的变化情况。对大鼠肝脏微粒体进行的放射免疫扩散分析表明，与年轻大鼠相比，衰老大鼠中细胞色素P - 450的1型和2型对β - 萘黄酮或苯巴比妥诱导的反应较差。β - 萘黄酮对衰老大鼠中3型、4型和5型的诱导效果比年轻大鼠差。然而，苯巴比妥对衰老大鼠中3型和4型的诱导效果比年轻大鼠好，但在年轻或衰老大鼠中均不诱导5型。虽然b型主要由苯巴比妥诱导，但β - 萘黄酮在衰老大鼠中能适度诱导b型。苯巴比妥在衰老大鼠和年轻大鼠中诱导b型的程度大致相同。在未处理的大鼠中，2型是主要形式，而1型和3型含量适中。通过对用β - 萘黄酮或苯巴比妥预处理的衰老和年轻大鼠微粒体中的细胞色素P - 450进行分离和部分纯化，证实了免疫扩散分析的结果。这些结果确定了大鼠衰老过程中细胞色素P - 450特定形式随年龄的变化。
