Marchetti Nicola, Guiochon Georges
Department of Chemistry, The University of Tennessee, Knoxville, TN 37996-1600, USA.
J Chromatogr A. 2007 Dec 28;1176(1-2):206-16. doi: 10.1016/j.chroma.2007.11.012. Epub 2007 Nov 12.
The performance of 5 and 15 cm long columns packed with shell particles (Halo, AMT) is compared in gradient elution separations of the tryptic digests of myoglobin and bovine serum albumin. The influences of the temperature and the mobile phase flow rate on the column efficiency for two peptides are discussed. The influences of this flow rate, of the temperature, and of the gradient slopes on the peak capacities are also considered. Peak capacities in excess of 400 were achieved in 6h with the longer column. Peak capacities of 200 could be achieved in 30 min with the shorter column.
在肌红蛋白和牛血清白蛋白胰蛋白酶消化产物的梯度洗脱分离中，比较了填充有壳颗粒（Halo、AMT）的5厘米和15厘米长色谱柱的性能。讨论了温度和流动相流速对两种肽的柱效的影响。还考虑了流速、温度和梯度斜率对峰容量的影响。使用较长的色谱柱在6小时内实现了超过400的峰容量。使用较短的色谱柱在30分钟内可实现200的峰容量。
