Marchetti Nicola, Fairchild Jacob N, Guiochon Georges
Department of Chemistry, The University of Tennessee, Knoxville, Tennessee 37996-1600, USA.
Anal Chem. 2008 Apr 15;80(8):2756-67. doi: 10.1021/ac7022662. Epub 2008 Mar 21.
The separation of the peptide digests of myoglobin and bovine serum albumin was performed with an off-line combination of two commercial, conventional HPLC columns. The first column was packed with a strong ion exchanger and eluted with a KCl gradient. The second column was packed with particles of C18-bonded silica and eluted with an acetonitrile gradient. The conditional peak capacities of the 2D separations achieved exceed 7000 under the experimental conditions investigated. This performance is achieved at the cost of an analysis time of the order of 28 hours. Possible improvements to the separation method described here are discussed.
使用两根商用常规高效液相色谱（HPLC）柱离线联用对肌红蛋白和牛血清白蛋白的肽消化产物进行分离。第一根柱填充强离子交换剂，用氯化钾梯度洗脱。第二根柱填充C18键合硅胶颗粒，用乙腈梯度洗脱。在所研究的实验条件下，二维分离实现的条件峰容量超过7000。这一性能是以约28小时的分析时间为代价实现的。本文讨论了对此处所述分离方法可能的改进。
