Raimundo J, Vale C, Duarte R, Moura I
National Institute for Agronomy and Fisheries Research - IPIMAR, Av. Brasília, 1449-006 Lisbon, Portugal.
Sci Total Environ. 2008 Feb 15;390(2-3):410-6. doi: 10.1016/j.scitotenv.2007.10.029. Epub 2007 Nov 26.
Concentrations of Zn, Cu, Cd and Pb and their sub-cellular distributions were determined in composite samples of digestive glands of the common octopus, Octopus vulgaris caught from two areas of the Portuguese coast characterised by contrasting metal contamination. Minor contents of Zn (1%), Cu (2%), Cd (6%) and Pb (7%) were found in the insoluble fraction, consisting of nuclei, mitochondria, lysosomes and microsome operationally separated from the whole digestive gland through a sequential centrifugation. A tendency for linear relationships between metal concentrations in nuclei, mitochondria, lysosomes and whole digestive gland was observed. These relationships suggest that despite low metal content organelles responded to the increasing accumulated metals, which means that detoxifying mechanism in cytosol was incomplete. Poorer correlations between microsome and whole digestive gland did not point to metal toxicity in the analysed compartments. However, the high accumulated Cd indicated that O. vulgaris is an important vehicle of this element to its predators in the coastal environment.
测定了从葡萄牙海岸两个具有不同金属污染特征的区域捕获的普通章鱼（Octopus vulgaris）消化腺复合样本中锌、铜、镉和铅的浓度及其亚细胞分布。通过连续离心从整个消化腺中操作分离出由细胞核、线粒体、溶酶体和微粒体组成的不溶部分，其中锌（1%）、铜（2%）、镉（6%）和铅（7%）含量较低。观察到细胞核、线粒体、溶酶体和整个消化腺中金属浓度之间存在线性关系趋势。这些关系表明，尽管细胞器中的金属含量较低，但它们对积累的金属增加做出了反应，这意味着细胞质中的解毒机制不完整。微粒体与整个消化腺之间较差的相关性并未表明所分析的区室中存在金属毒性。然而，高积累的镉表明普通章鱼是这种元素在沿海环境中向其捕食者传递的重要载体。
