IPIMAR-National Institute of Biological Resources, Av. Brasília, 1449-006 Lisbon, Portugal.
Ecotoxicol Environ Saf. 2010 Oct;73(7):1543-7. doi: 10.1016/j.ecoenv.2010.07.034. Epub 2010 Aug 16.
The alkaline comet assay has been employed for the first time to estimate the basal DNA damage in the digestive gland, gills, kidney and gonads of Octopus vulgaris. Octopuses were captured in two coastal areas adjacent to the cities of Matosinhos (N) and Olhão (S), Portugal. The area of Matosinhos is influenced by discharges of the Douro River, city of Porto, industries and intensive agriculture, while Olhão is an important fisheries port. Previous works point to contrasting metal availability in the two coastal areas. Among the analysed tissues digestive gland presented the highest levels of Zn, Cu, Cd and Pb. Tissues of specimens from Matosinhos exhibited high levels of Cd and from Olhão enhanced Pb concentrations. The DNA damages in digestive gland, gills and kidney were more accentuated in specimens from Matosinhos than from Olhão, suggesting a stronger effect of contaminants. Elevated strand breakages were registered in digestive gland, recognised for its ability to store and detoxify accumulated metals. The DNA damages in kidney, gills and gonads were lower, reflecting reduced metal accumulation or efficient detoxification. The broad variability of damages in the three tissues may also mirror tissue function, specific defences to genotoxicants and cell-cycle turnover.
碱性彗星试验首次被用于估计普通章鱼的消化腺、鳃、肾和性腺中的基础 DNA 损伤。章鱼在葡萄牙马托西纽什（N）和奥良（S）两个沿海地区被捕获。马托西纽什地区受到杜罗河、波尔图市、工业和集约化农业的排放影响，而奥良是一个重要的渔业港口。先前的研究表明，这两个沿海地区的金属可用性存在差异。在所分析的组织中，消化腺的锌、铜、镉和铅含量最高。来自马托西纽什的标本组织表现出高浓度的镉，而来自奥良的标本则显示出增强的铅浓度。与来自奥良的标本相比，来自马托西纽什的标本的消化腺、鳃和肾中的 DNA 损伤更为明显，这表明污染物的影响更强。在具有储存和解毒积累金属能力的消化腺中，检测到了明显的链断裂。在肾、鳃和性腺中，DNA 损伤较低，这反映了金属积累的减少或有效的解毒作用。这三种组织中的损伤的广泛变异性也可能反映了组织功能、对遗传毒物的特定防御和细胞周期更新。
