Martin H, Martin R
Aktuelle Gerontol. 1977 May;7(5):247-52.
The labelling behaviour of RNA fractions in rat liver after application of 32P-orthophosphate and 14C-orotate in vivo in young (3-5 months) and old animals (20 months) was presented. Whereas the tracer incorporation in old animals 60 minutes after 32P-application seems to be decreased we observed increased specific activities of nuclear RNA 30 and 60 minutes after application of 14C-orotate i.p. - Measuring of RNA synthesis in isolated cell nuclei after in vivo stimulation by cortisol (2 mg/100 g body weight) showed a stronger and longer continued stimulation in young animals as in old both in the Mn++ system and in the Mg++ system. When we compared the young with the old controls the rate of synthesis was increased in the old controls. The results support observations on changed regulations of the transcription of genetic informations in the age.
本文展示了在幼年（3 - 5个月）和老年（20个月）大鼠体内应用32P - 正磷酸盐和14C - 乳清酸后，大鼠肝脏中RNA组分的标记行为。虽然在应用32P后60分钟，老年动物中示踪剂的掺入似乎减少，但我们观察到腹腔注射14C - 乳清酸后30分钟和60分钟，核RNA的比活性增加。在体内用皮质醇（2mg/100g体重）刺激后，对分离细胞核中RNA合成的测量表明，在Mn++系统和Mg++系统中，幼年动物比老年动物受到更强且持续时间更长的刺激。当我们将幼年动物与老年对照动物进行比较时，老年对照动物的合成速率增加。这些结果支持了关于年龄增长过程中遗传信息转录调控变化的观察结果。
