Ivanovskiĭ R N, Rodova N A
Mikrobiologiia. 1976 Mar-Apr;45(2):197-200.
Assimilation of oxygen by the cells of Rhodopseudomonas palustris grown in the light and in the darkness is stimulated by p-chlorocarbonylcyanidephanylhydrazone, suggesting respiration coupled to phosphorylatin. Membranes of the cells grown in the light are capable of oxidative phosphorylation in the course of electron transport from NADH and succinate to O2 with P/O being 0.03 and 0.20, respectively. Membranes of the cells grown in the darkness in aerobic conditions are capable of oxidative phosphorylation in the presence of NADH, succinate and ascorbate + phenazinemetasulphate with P/O being 0.25, 0.40 and 0.06, respectively. Phosphorylation during oxidation of ascorbate + phenazinemetasulphate in the cells grown in the darkness suggests that the third point of coupling has appeared as a result of synthesis of cytochrome a. There are only minor differences in phosphorylation in the membranes of the cells grown in the light and in the darkness, as was shown by its susceptibility to p-chlorocarbonylcyanidephenyl-hydrazone, oligomycin, antimycin A and cyanide.
在光照和黑暗条件下生长的沼泽红假单胞菌细胞对氧的同化作用受对氯羰基氰苯腙刺激，这表明呼吸作用与磷酸化作用相偶联。在光照下生长的细胞的膜，在从NADH和琥珀酸到O₂的电子传递过程中能够进行氧化磷酸化，P/O分别为0.03和0.20。在需氧条件下于黑暗中生长的细胞的膜，在存在NADH、琥珀酸和抗坏血酸 + 吩嗪硫酸甲酯时能够进行氧化磷酸化，P/O分别为0.25、0.40和0.06。在黑暗中生长的细胞中抗坏血酸 + 吩嗪硫酸甲酯氧化过程中的磷酸化作用表明，细胞色素a的合成导致了第三个偶联点的出现。光照和黑暗条件下生长的细胞的膜在磷酸化方面只有微小差异，这一点通过其对对氯羰基氰苯腙、寡霉素、抗霉素A和氰化物的敏感性得以体现。
