[鼠麻风杆菌中的氧化磷酸化]

[Oxidative phosphorylation in Mycobacterium lepraemurium].

作者信息

Ishaque M, Adapoe C, Kato L

出版信息

Rev Can Biol. 1980 Dec;39(4):219-23.

Abstract

The generation of ATP by cell-free extracts of Mycobacterium lepraemurium isolated from Sprague-Dawley rats was investigated. Cell-free preparations catalyzed phosphorylation coupled to the oxidation of NADH and succinate yielding P/O ratios of 0.6 and 0.4, respectively. Ascorbate oxidation did not result in ATP formation. The oxidative phosphorylation was uncoupled by 2,4- dinitrophenol and pentachlorophenol. Phosphate esterification coupled to NADH oxidation was inhibited by rotenone which had no effect on ATP synthesis associated with succinate oxidation. Antimycin A and cyanide completely inhibited phosphorylation coupled to the oxidation of NADH or succinate.

摘要

对从斯普拉格-道利大鼠分离出的鼠麻风分枝杆菌无细胞提取物产生三磷酸腺苷（ATP）的情况进行了研究。无细胞制剂催化与烟酰胺腺嘌呤二核苷酸（NADH）和琥珀酸氧化偶联的磷酸化反应，磷氧比（P/O）分别为0.6和0.4。抗坏血酸氧化未导致ATP形成。氧化磷酸化被2,4-二硝基苯酚和五氯苯酚解偶联。与NADH氧化偶联的磷酸酯化反应被鱼藤酮抑制，而鱼藤酮对与琥珀酸氧化相关的ATP合成没有影响。抗霉素A和氰化物完全抑制与NADH或琥珀酸氧化偶联的磷酸化反应。