Frohn J T, Knapp H F, Stemmer A
Opt Lett. 2001 Jun 1;26(11):828-30. doi: 10.1364/ol.26.000828.
A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A passband with a high cutoff frequency throughout reciprocal space can be achieved by illumination of the object with spatially harmonic excitation patterns generated by the interference of two collimated laser beams. Theoretical calculations show an almost isotropic point-spread function with a FWHM near 100 nm.
提出了一种提高荧光显微镜横向和轴向分辨率的方法。通过用由两束准直激光束干涉产生的空间谐波激发图案照射物体，可以在整个倒易空间中实现具有高截止频率的通带。理论计算表明，其具有半高宽接近100nm的几乎各向同性的点扩散函数。
