Luo Fengshan, Sun Haiying, Geng Tao, Qi Nianmin
School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P.R. China.
Biotechnol Lett. 2008 Apr;30(4):645-9. doi: 10.1007/s10529-007-9604-2. Epub 2007 Nov 27.
Taguchi's L8 experimental design was firstly applied to identify critical parameters affecting the efficiency of bridge formation between confluent and fresh microcarriers in bead-to-bead transfer of Vero cells. Statistical analysis indicated that stirring time, rest time and the interactions between stirring time and rest time, stirring speed and rest time had significant effects. Subsequently, a two-stage cell transfer process was performed successfully, in which an efficiency of bridge formation (15.3 +/- 1.7%) obtained from 8 h intermittent agitation made percentage of bare microcarriers decrease quickly from 66.7% to 2.0 +/- 0.8%.
田口的L8实验设计首次用于确定在Vero细胞珠对珠转移中影响汇合微载体与新鲜微载体之间桥接形成效率的关键参数。统计分析表明，搅拌时间、静置时间以及搅拌时间与静置时间、搅拌速度与静置时间之间的相互作用具有显著影响。随后，成功进行了两阶段细胞转移过程，其中通过8小时间歇搅拌获得的桥接形成效率（15.3±1.7%）使裸微载体的百分比从66.7%迅速降至2.0±0.8%。
