Lim H S, Han B K, Kim J H, Peshwa M V, Hu W S
Department of Biotechnology, Korea Advanced Institute of Science and Technology, Taejon.
Biotechnol Prog. 1992 Nov-Dec;8(6):486-93. doi: 10.1021/bp00018a003.
Vero and HepG2 cells were cultivated on macroporous gelatin microcarriers prepared by the calcium carbonate inclusion method. Cell attachment to these microcarriers was slow. For HepG2 cells the subsequent growth was poor. Modification of the microcarriers by incorporation of (diethylamino)ethyl-HCl improved HepG2 attachment and subsequent growth. Optical sectioning with confocal microscopy allowed visualization of the distribution of cells within microcarriers. In most microcarriers, cells were found to preferentially populate regions close to the external surface and some cavities in the interior. Despite the incomplete occupancy of the interior of the microcarriers, high cell concentrations were achieved.
将Vero细胞和HepG2细胞培养于通过碳酸钙包埋法制备的大孔明胶微载体上。细胞附着于这些微载体的过程较为缓慢。对于HepG2细胞而言，其后续生长较差。通过掺入（二乙氨基）乙基盐酸盐对微载体进行修饰，改善了HepG2细胞的附着及后续生长。利用共聚焦显微镜进行光学切片能够观察到细胞在微载体内的分布情况。在大多数微载体中，发现细胞优先聚集在靠近外表面的区域以及内部的一些腔隙中。尽管微载体内部未被完全占据，但仍实现了高细胞浓度。
