Zhang Jie, Hoogmartens Jos, Van Schepdael Ann
Laboratory for Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Leuven, Belgium.
Electrophoresis. 2008 Jan;29(1):56-65. doi: 10.1002/elps.200700475.
This review, as a continuation of two earlier reports, gives an overview of the recent developments, over the period from 2005 until now, in the use of electrophoretically mediated microanalysis (EMMA) methodology for the on-line study of enzymatic reaction and derivatization. The article is divided into two parts: (i) on-line enzymatic reaction by EMMA and (ii) on-line derivatization by EMMA. Following a brief introduction, a literature overview on enzymatic reaction is provided. The second part starts with an introduction of the purpose of derivatization and the nomenclature used in the area of in-capillary derivatization based on EMMA. The development of more integrated analytical platform that combines in-capillary derivatization and sample preconcentration is discussed. Reported derivatization procedures are summarized.
本综述作为前两份报告的延续，概述了2005年至今电泳介导微分析（EMMA）方法在酶促反应和衍生化在线研究中的最新进展。本文分为两部分：（i）EMMA在线酶促反应和（ii）EMMA在线衍生化。在简要介绍之后，提供了关于酶促反应的文献综述。第二部分首先介绍了衍生化的目的以及基于EMMA的毛细管内衍生化领域中使用的命名法。讨论了结合毛细管内衍生化和样品预浓缩的更集成分析平台的开发。总结了报道的衍生化程序。
