Paulesu L, King A, Loke Y W, Cintorino M, Bellizzi E, Boraschi D
Institute of General Physiology, University of Siena, Italy.
Lymphokine Cytokine Res. 1991 Dec;10(6):443-8.
Many reports show that interleukin 1 (IL-1) is produced by mouse and human placenta but the cell type that is responsible for this production has yet to be identified. For this reason we attempted to localize IL-1 alpha and IL-1 beta directly on formalin-fixed paraffin-embedded normal human placentae at different stages of pregnancy using immunohistochemical techniques and specific antibodies. The results obtained show that both IL-1 forms are localized to villous syncytiotrophoblast and to extravillous trophoblast, while villous cytotrophoblast and cytotrophoblast columns are unreactive. A gradual decrease of reactivity was observed with increasing gestation age for both IL-1 forms, but the staining for IL-1 beta was in all sections higher than for IL-1 alpha. Although the physiological role of IL-1 in pregnancy has yet to be established, the presence of this cytokine in the cells facing maternal blood and tissues suggests a possible involvement in the immunoregulation of fetal acceptance.
许多报告显示，白细胞介素1（IL-1）由小鼠和人类胎盘产生，但负责这种产生的细胞类型尚未确定。因此，我们试图使用免疫组织化学技术和特异性抗体，将IL-1α和IL-1β直接定位在不同妊娠阶段的福尔马林固定石蜡包埋的正常人类胎盘上。获得的结果表明，两种IL-1形式都定位于绒毛合体滋养层和绒毛外滋养层，而绒毛细胞滋养层和细胞滋养层柱无反应。随着妊娠年龄的增加，两种IL-1形式的反应性逐渐降低，但IL-1β在所有切片中的染色都高于IL-1α。尽管IL-1在妊娠中的生理作用尚未确定，但这种细胞因子在面对母体血液和组织的细胞中的存在表明它可能参与了胎儿接受的免疫调节。
