Pedrero Zoyne, Madrid Yolanda, Hartikainen Helinä, Cámara Carmen
Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.
J Agric Food Chem. 2008 Jan 9;56(1):266-71. doi: 10.1021/jf072266w. Epub 2007 Nov 29.
The protective effect of selenium against the cadmium-induced oxidative effect in broccoli ( Brassica oleracea) plants was studied. Plants grown in hydroponic culture were supplied with selenium [as Se(IV)] and cadmium [as Cd(II)], individually or simultaneously. Cadmium accumulation in roots was noticeably higher than in the aerial parts of the plants, and this effect was even more acute when selenium was simultaneously added. Cadmium phytotoxicity was evidenced by an increase in the malondialdehyde (MDA) concentration in the roots and a decrease of photosynthetic pigment and tocopherol concentration in the aerial parts of the plant. The simultaneous addition of selenium alleviated cadmium-induced stress in the roots after 40 days of exposition. In the leaves, a more remarkable decrease of tocopherol and chlorophyll concentration was observed in the cadmium-enriched plants after 10 days of exposure. The results provided evidence that selenium supplementation helps the plant to minimize the cadmium oxidant effect. Tocopherol concentration in broccoli fruit of cadmium-supplied plants was not affected in comparison to control. However, the proportion of alpha-tocopherol increases with the addition of selenium. This response is important not only for the protective effect against oxidative damage in the plant but also in terms of human nutrition.
研究了硒对西兰花（甘蓝）植株中镉诱导的氧化作用的保护效应。在水培条件下生长的植株分别或同时供应硒[以硒（IV）形式]和镉[以镉（II）形式]。镉在根部的积累明显高于植株地上部分，当同时添加硒时这种效应更为明显。镉的植物毒性表现为根部丙二醛（MDA）浓度增加以及植株地上部分光合色素和生育酚浓度降低。暴露40天后，同时添加硒减轻了镉对根部的诱导胁迫。在叶片中，暴露10天后，在富含镉的植株中观察到生育酚和叶绿素浓度有更显著的降低。结果表明，补充硒有助于植物将镉的氧化效应降至最低。与对照相比，供应镉的植株西兰花果实中的生育酚浓度未受影响。然而，随着硒的添加，α-生育酚的比例增加。这种反应不仅对植物抗氧化损伤的保护作用很重要，对人类营养而言也很重要。
