Wang Wenxi, Zhang Fan, Liu Dapu, Chen Kai, Du Bin, Qiu Xianjin, Xu Jianlong, Xing Danying
College of Economy and Management, Hubei University of Technology, Wuhan, China.
MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, China.
Front Genet. 2022 Oct 13;13:1007896. doi: 10.3389/fgene.2022.1007896. eCollection 2022.
High selenium (Se) and low cadmium (Cd) and arsenic (As) contents in rice grains were good for human health. The genetic basis and relationship of Se, Cd and As concentrations in rice grains are still largely unknown. In the present study, large variations were observed in Se, Cd and As concentrations in brown and milled rice in normal and Se treatment conditions in 307 rice accessions from 3K Rice Genomes Project. Se fertilizer treatment greatly increased Se concentrations but had no obvious changes in concentrations of Cd and As both in brown and milled rice. Total of 237 QTL were identified for Se, Cd and As concentrations in brown and milled rice in normal and Se treatment conditions as well as ratio of concentrations under Se treatment to normal conditions. Only 19 QTL (13.4%) were mapped for concentrations of Se and Cd, Se and As, and Se, Cd and As in the same or adjacent regions, indicating that most Se concentration QTL are independent of Cd and As concentration QTL. Forty-three favorable alleles were identified for 40 candidate genes by gene-based association study and haplotype analysis in 14 important QTL regions. Se-enriched rice variety will be developed by pyramiding favorable alleles at different Se QTL and excluding undesirable alleles at Cd and As QTL, or combining favorable alleles at Se QTL with the alleles at Se-sensitive QTL by marker-assisted selection.
水稻籽粒中高硒（Se）、低镉（Cd）和低砷（As）含量对人体健康有益。水稻籽粒中硒、镉和砷含量的遗传基础及相互关系仍 largely 未知。在本研究中，对来自 3K 水稻基因组计划的 307 份水稻种质，在正常和施硒处理条件下糙米和精米中的硒、镉和砷含量进行了测定，观察到含量存在较大变异。施硒处理显著提高了糙米和精米中的硒含量，但镉和砷含量无明显变化。共鉴定出 237 个控制糙米和精米在正常和施硒处理条件下硒、镉和砷含量以及施硒处理与正常条件下含量比值的 QTL。仅 19 个 QTL（13.4%）定位在硒和镉、硒和砷、硒、镉和砷含量的相同或相邻区域，表明大多数硒含量 QTL 独立于镉和砷含量 QTL。通过基于基因的关联分析和单倍型分析，在 14 个重要 QTL 区域为 40 个候选基因鉴定出 43 个有利等位基因。将通过在不同硒 QTL 上聚合有利等位基因并排除镉和砷 QTL 上的不良等位基因，或通过标记辅助选择将硒 QTL 上的有利等位基因与硒敏感 QTL 上的等位基因相结合，来培育富硒水稻品种。
