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Cell-penetrating peptides: mechanism and kinetics of cargo delivery.细胞穿透肽：货物递送的机制与动力学
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Circular dichroism spectra of short, fixed-nucleus alanine helices.短的、固定核的丙氨酸螺旋的圆二色光谱。
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Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski's equality.在对雅尔津斯基等式的实验测试中，从非平衡测量中获取的平衡信息。
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具有构象开关的被动水脂肽转运体：从单分子探针到细胞检测

Passive water-lipid peptide translocators with conformational switches: from single-molecule probe to cellular assay.

作者信息

Fernández Ariel, Crespo Alejandro, Blau Axel

机构信息

Department of Bioengineering, Rice University, Houston, Texas 77005, USA.

出版信息

J Phys Chem B. 2007 Dec 20;111(50):13987-92. doi: 10.1021/jp074479u. Epub 2007 Nov 29.

DOI:10.1021/jp074479u
PMID:18044863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2528833/
Abstract

Peptide design for unassisted passive water-lipid translocation remains a challenge, notwithstanding its importance for drug delivery. We introduce a design paradigm based on conformational switches operating as passive translocation vehicles. The interfacial behavior of the molecular prototype, probed in single-molecule AFM experiments, reveals a near-barrierless translocation. The associated free-energy agrees with mesoscopic measurements, and the in vitro behavior is quantitatively reproduced in cellular assays. The prototypes herald the advent of novel nanobiomaterials for passive translocation.

摘要

尽管肽设计对于药物递送很重要，但用于无辅助被动水-脂转运的肽设计仍然是一个挑战。我们引入了一种基于作为被动转运载体的构象开关的设计范式。在单分子原子力显微镜实验中探测的分子原型的界面行为揭示了近乎无障碍的转运。相关的自由能与介观测量结果一致，并且在细胞试验中定量再现了体外行为。这些原型预示着用于被动转运的新型纳米生物材料的出现。