Oshima Tatsuya, Baba Yoshinari, Shimojo Kojiro, Goto Masahiro
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1, Gakuen Kibanadai Nishi, Miyazaki, 889-2192, Japan.
Curr Drug Discov Technol. 2007 Dec;4(4):220-8. doi: 10.2174/157016307783220512.
A macrocyclic calix[6]arene carboxylic acid derivative is found to extract lysine-rich protein cytochrome c from aqueous media into organic media through the complexation between the calixarene molecules and lysine residues on the surface of the protein. This article summarizes both the mechanism of protein extraction by the calixarene as well as the potential applications of the extraction process. The extraction process can be used for the purification of proteins through selective extraction and back-extraction under optimized conditions. On the other hand, the extracted protein exhibits enzymatic activity in organic media. The formation of a supramolecular complex by recognizing the residues on a protein surface can be construed as a novel recognition and/or modification method for biomacromolecules.
发现一种大环杯[6]芳烃羧酸衍生物通过杯芳烃分子与蛋白质表面赖氨酸残基之间的络合作用，将富含赖氨酸的蛋白质细胞色素c从水相介质萃取到有机相介质中。本文总结了杯芳烃萃取蛋白质的机制以及萃取过程的潜在应用。在优化条件下，该萃取过程可通过选择性萃取和反萃取用于蛋白质的纯化。另一方面，萃取得到的蛋白质在有机介质中表现出酶活性。通过识别蛋白质表面的残基形成超分子复合物可被视为一种用于生物大分子的新型识别和/或修饰方法。
