Shidaifat F
Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110 Jordan.
J Vet Med A Physiol Pathol Clin Med. 2007 Dec;54(10):549-52. doi: 10.1111/j.1439-0442.2007.00983.x.
Keratinocyte growth factor (KGF) and fibroblast growth factor 10 (FGF-10) are stromal-derived growth factors that interact with their epithelial FGFR2 receptors to mediate stromal--epithelial cell interaction within the prostate gland. This study was conducted to compare the development-related mRNA expression of KGF, FGF-10 and their receptor FGFR2 in immature and mature canine prostate glands. In addition, their expression levels were correlated with the differentiation of stromal cells using vimentin as a mesenchymal cell marker. Quantitative mRNA expression was assessed by real-time polymerase chain reaction (PCR) and the results were expressed as relative mRNA expression of the target gene, which was normalized to the GAPDH reference gene. mRNA analysis revealed a differential expression of KGF, FGF-10 and FGFR2 receptor by the prostate glands of immature and mature dogs. The results showed a 7.3- and 9-fold decrease in mRNA expression of KGF and FGF-10 by mature and immature prostate glands respectively. However, there was no significant change in FGFR2 receptor mRNA expression by mature or immature prostate glands. This downregulation of KGF and FGF-10 expression was associated with a 15-fold decrease in vimentin expression. These results indicate that KGF and FGF-10 expression varied according to the differentiation status of stromal cells and might reflect differential developmental requirements of immature and mature canine prostate glands.
角质形成细胞生长因子（KGF）和成纤维细胞生长因子10（FGF - 10）是基质来源的生长因子，它们与其上皮FGFR2受体相互作用，以介导前列腺内基质 - 上皮细胞的相互作用。本研究旨在比较未成熟和成熟犬前列腺中KGF、FGF - 10及其受体FGFR2与发育相关的mRNA表达。此外，使用波形蛋白作为间充质细胞标志物，将它们的表达水平与基质细胞的分化相关联。通过实时聚合酶链反应（PCR）评估定量mRNA表达，结果表示为目标基因的相对mRNA表达，并将其标准化为GAPDH参考基因。mRNA分析显示，未成熟和成熟犬的前列腺中KGF、FGF - 10和FGFR2受体存在差异表达。结果表明，成熟和未成熟前列腺中KGF和FGF - 10的mRNA表达分别下降了7.3倍和9倍。然而，成熟或未成熟前列腺中FGFR2受体mRNA表达没有显著变化。KGF和FGF - 10表达的这种下调与波形蛋白表达下降15倍相关。这些结果表明，KGF和FGF - 10的表达根据基质细胞的分化状态而变化，可能反映了未成熟和成熟犬前列腺不同的发育需求。
