Canatan H, Chang W Y, Sugimoto Y, Shidaifat F, Kulp S K, Brueggemeier R W, Lin Y C
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, 43210-1092, USA.
DNA Cell Biol. 1996 Mar;15(3):247-54. doi: 10.1089/dna.1996.15.247.
cDNA encoding the canine keratinocyte growth factor (KGF) was cloned from normal canine prostate tissue. The authentic canine KGF cDNA sequence, 686 bp in length, spans the protein-coding region and 88 bp of the 5' and 19 bp of the 3' untranslated regions of canine KGF. The predicted amino acid sequence of canine KGF is composed of 194 amino acid residues. Canine KGF exhibits highest homology with the human KGF cDNA and amino acid sequences (95.8% and 97.4%, respectively), while it demonstrates the lowest homology with the rat sequences at 88.0% and 92.3%, respectively. The degrees of homology with mouse cDNA and amino acid sequences are 91.8% and 95.9%, respectively. By using RNase protection assay, KGF was shown to be expressed in normal prostate tissues of both mature and young (5-month-old) dogs. In vitro, the recombinant canine KGF has mitogenic activity on cultured canine epithelial cells, whereas it has no effect on cultured canine prostatic stromal cells. This novel canine KGF cDNA may be a valuable tool in the study of human benign prostatic hyperplasia using the canine prostatic as a model.
从正常犬前列腺组织中克隆出编码犬角质形成细胞生长因子（KGF）的cDNA。真实的犬KGF cDNA序列长度为686 bp，跨越蛋白质编码区以及犬KGF 5'端的88 bp和3'端非翻译区的19 bp。犬KGF的预测氨基酸序列由194个氨基酸残基组成。犬KGF与人类KGF cDNA和氨基酸序列的同源性最高（分别为95.8%和97.4%），而与大鼠序列的同源性最低，分别为88.0%和92.3%。与小鼠cDNA和氨基酸序列的同源性程度分别为91.8%和95.9%。通过核糖核酸酶保护试验表明，KGF在成年犬和幼犬（5个月大）的正常前列腺组织中均有表达。在体外，重组犬KGF对培养的犬上皮细胞具有促有丝分裂活性，而对培养的犬前列腺基质细胞没有影响。这种新的犬KGF cDNA可能是利用犬前列腺作为模型研究人类良性前列腺增生的有价值工具。
