Li Xiaojing, Liu Jia, Davey Megan, Duce Suzanne, Jaberi Neema, Liu Gang, Davidson Gemma, Tenent Seaneen, Mahood Ruth, Brown Phoebe, Cunningham Craig, Bain Andrew, Beattie Kevin, McDonald Laura, Schmidt Katy, Towers Matthew, Tickle Cheryll, Chudek Sandy
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, UK.
J Anat. 2007 Dec;211(6):798-809. doi: 10.1111/j.1469-7580.2007.00825.x.
Chick embryos are useful models for probing developmental mechanisms including those involved in organogenesis. In addition to classic embryological manipulations, it is possible to test the function of molecules and genes while the embryo remains within the egg. Here we define conditions for imaging chick embryo anatomy and for visualising living quail embryos. We focus on the developing limb and describe how different tissues can be imaged using micro-magnetic resonance imaging and this information then synthesised, using a three-dimensional visualisation package, into detailed anatomy. We illustrate the potential for micro-magnetic resonance imaging to analyse phenotypic changes following chick limb manipulation. The work with the living quail embryos lays the foundations for using micro-magnetic resonance imaging as an experimental tool to follow the consequences of such manipulations over time.
鸡胚是探究发育机制（包括器官发生相关机制）的有用模型。除了经典的胚胎学操作外，在胚胎仍处于卵内时就可以测试分子和基因的功能。在这里，我们定义了对鸡胚解剖结构进行成像以及对活鹌鹑胚胎进行可视化的条件。我们聚焦于发育中的肢体，并描述如何使用显微磁共振成像对不同组织进行成像，然后利用三维可视化软件包将这些信息整合为详细的解剖结构。我们阐述了显微磁共振成像在分析鸡胚肢体操作后表型变化方面的潜力。对活鹌鹑胚胎的研究为将显微磁共振成像用作实验工具以追踪此类操作随时间产生的后果奠定了基础。
