Duval S M, McEwan N R, Graham R C, Wallace R J, Newbold C J
The Institute of Rural Science, University of Wales, Aberystwyth, Wales, UK.
J Appl Microbiol. 2007 Dec;103(6):2132-41. doi: 10.1111/j.1365-2672.2007.03455.x.
To investigate the mode of action of a blend of essential oil compounds on the colonization of starch-rich substrates by rumen bacteria.
Starch-rich substrates were incubated, in nylon bags, in the rumen of sheep organized in a 4 x 4 latin square design and receiving a 60:40 silage : concentrate diet. The concentrate was either high or low in crude protein, and the diet was supplemented or not with a commercial blend of essential oil compounds (110 mg per day). The total genomic DNA was extracted from the residues in the bags. The total eubacterial DNA was quantified by real-time PCR and the proportion of Ruminobacter amylophilus, Streptococcus bovis and Prevotella bryantii was determined. Neither the supplementation with essential oil compounds nor the amount of crude protein affected the colonization of the substrates by the bacteria quantified. However, colonization was significantly affected by the substrate colonized.
The effect of essential oils on the colonization of starch-rich substrates is not mediated through the selective inhibition of R. amylophilus.
This study enhances our understanding of the colonization of starch-rich substrates, as well as of the mode of action of the essential oils as rumen manipulating agents.
研究精油化合物混合物对瘤胃细菌在富含淀粉底物上定殖的作用方式。
将富含淀粉的底物装入尼龙袋，置于采用4×4拉丁方设计且饲喂60:40青贮料:精料日粮的绵羊瘤胃中。精料的粗蛋白含量有高有低，日粮添加或不添加市售精油化合物混合物（每天110毫克）。从袋中的残留物中提取总基因组DNA。通过实时荧光定量PCR对总真细菌DNA进行定量，并测定嗜淀粉瘤胃杆菌、牛链球菌和布氏普雷沃氏菌的比例。精油化合物的添加和粗蛋白含量均未影响所定量细菌在底物上的定殖。然而，定殖受到所定殖底物的显著影响。
精油对富含淀粉底物定殖的影响并非通过对嗜淀粉瘤胃杆菌的选择性抑制介导。
本研究增进了我们对富含淀粉底物定殖以及精油作为瘤胃调控剂作用方式的理解。
