Paz Z, Burdman S, Gerson U, Sztejnberg A
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
J Appl Microbiol. 2007 Dec;103(6):2570-9. doi: 10.1111/j.1365-2672.2007.03512.x.
The fungus Meira geulakonigii has been shown to reduce populations of citrus rust mite (CRM; Phyllocoptruta oleivora) on citrus leaves and fruits, in both the field and laboratory. However, attempts to isolate the fungus from leaves and fruits have been unsuccessful. The aims of this study were therefore to determine whether M. geulakonigii is a citrus endophyte, and to assess possible mechanisms involved in its mite-antagonist activity.
A quantitative real-time PCR and regular PCR approaches were developed to detect M. geulakonigii in both the field and laboratory. The fungus was detected throughout. Different methods revealed that M. geulakonigii is an endophyte, which colonizes both the peel of grapefruits. Applications of conidia protected the grapefruits against CRM, and fungal secretions extracted from growth media caused 100% CRM mortality.
Meira geulakonigii is a beneficial endophyte of grapefruits that colonizes the fruit's peel, and protects it from CRM.
Findings from this study demonstrate the endophytic nature of M. geulakonigii in its interaction with grapefruits. In addition, a molecular approach was developed to specifically detect the fungus inside the grapefruit peel. This approach can be used to assess the natural occurrence of M. geulakonigii in grapefruit.
在田间和实验室中均已证明，古氏梅拉真菌可减少柑橘叶片和果实上的柑橘锈螨（Phyllocoptruta oleivora）数量。然而，从叶片和果实中分离该真菌的尝试均未成功。因此，本研究的目的是确定古氏梅拉真菌是否为柑橘内生菌，并评估其对螨的拮抗活性所涉及的可能机制。
开发了定量实时PCR和常规PCR方法，用于在田间和实验室中检测古氏梅拉真菌。在整个过程中均检测到了该真菌。不同方法表明，古氏梅拉真菌是一种内生菌，它定殖于葡萄柚的果皮。分生孢子的应用保护葡萄柚免受柑橘锈螨侵害，从生长培养基中提取的真菌分泌物导致柑橘锈螨100%死亡。
古氏梅拉真菌是葡萄柚的一种有益内生菌，定殖于果实的果皮，并保护其免受柑橘锈螨侵害。
本研究结果证明了古氏梅拉真菌在与葡萄柚相互作用中的内生性质。此外，还开发了一种分子方法来特异性检测葡萄柚果皮内的真菌。该方法可用于评估葡萄柚中古氏梅拉真菌的自然存在情况。
