Int J Cardiol. 2009 Feb 6;132(1):135-7. doi: 10.1016/j.ijcard.2007.07.143. Epub 2007 Nov 28.
Diastolic dysfunction is a major component of hypertensive cardiomyopathy contributing to a progressive evolution towards overt heart failure. To establish an experimental model that could mimic the human clinical pattern, we standardized the surgery in one-kidney, one-clip Goldblatt (1K,1C) rabbits and characterized their hypertensive cardiopathy by echocardiography. Five weeks after placement of a stenotic string around the left renal artery and removal of the right kidney, arterial pressure was measured and an echocardiography performed in conscious animals. An hypertrophic cardiopathy associated with hypertension and a primary trouble of the LV relaxation was observed. This trouble was characterized by a reversion of E/A and Ea/Aa ratios and an increase of the isovolumic relaxation time and Tau index, without augmentation of left ventricular filling pressures. We show for the first time, in this experimental model, a diastolic dysfunction pattern close to the human one. Moreover, echocardiography in a conscious state gives the opportunity to use this model for future chronic pharmacological studies.
舒张功能障碍是高血压性心肌病的主要组成部分，可导致明显心力衰竭的逐渐进展。为了建立一种能够模拟人类临床模式的实验模型，我们对单侧肾动脉狭窄加单侧肾切除的 Goldblatt（1K,1C）兔的手术进行了标准化，并通过超声心动图对其高血压性心肌病进行了特征描述。在将狭窄的丝线环绕左肾动脉并切除右肾 5 周后，在清醒动物中测量动脉压并进行超声心动图检查。观察到与高血压相关的肥厚性心肌病和 LV 松弛的原发性障碍。这种障碍的特征是 E/A 和 Ea/Aa 比值的反转，以及等容松弛时间和 Tau 指数的增加，而左心室充盈压没有增加。我们首次在该实验模型中观察到与人类非常相似的舒张功能障碍模式。此外，在清醒状态下进行超声心动图检查为未来的慢性药理学研究提供了使用该模型的机会。
