Holanda Pinto S A, Pinto L M S, Cunha G M A, Chaves M H, Santos F A, Rao V S
Department of Physiology and Pharmacology, Post-Graduation Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceara, POB 3157, Rua Cel Nunes de Melo, 1127, Porangabussu, 60430-270, Fortaleza, CE, Brazil.
Inflammopharmacology. 2008 Feb;16(1):48-52. doi: 10.1007/s10787-007-1609-x.
This study was aimed to evaluate the anti-inflammatory potential of triterpene alpha, beta-amyrin in rats on acute phase periodontitis. Periodontitis was induced by ligature placement around the maxillary right second molar tooth. Rats (n = 8/group) were pretreated with alpha, beta-amyrin (5 and 10 mg/kg, p. o.), two hours before the induction of periodontal inflammation. Sham-operated and positive controls (lumiracoxib and dexamethasone) were included. Six hours later, plasma levels of TNF-alpha were analysed. Rats were sacrificed at 24 h, and the gingival tissue analysed for myeloperoxidase (MPO) and thiobarbituric acid-reactive substances (TBARS), as measures of neutrophil influx and lipid-peroxidation, respectively alpha, beta-Amyrin as well as dexamethasone significantly inhibited the periodontitis-associated increases of TNF-alpha, and the gingival MPO and TBARS. alpha, beta-Amyrin effect was more prominent at 5 mg/kg. Lumiracoxib manifested varied influence on the studied parameters. These results provide evidence to show that alpha, beta-Amyrin retards acute inflammation in rat model of periodontitis and warrant further study on its efficacy to prevent chronic periodontitis-associated bone loss.
本研究旨在评估三萜类化合物α、β-香树脂醇对大鼠急性期牙周炎的抗炎潜力。通过在上颌右侧第二磨牙周围放置结扎线诱导牙周炎。在诱导牙周炎症前两小时，大鼠（每组n = 8）用α、β-香树脂醇（5和10 mg/kg，口服）进行预处理。设置假手术组和阳性对照组（氯美昔布和地塞米松）。六小时后，分析血浆中肿瘤坏死因子-α（TNF-α）水平。24小时后处死大鼠，分析牙龈组织中的髓过氧化物酶（MPO）和硫代巴比妥酸反应性物质（TBARS），分别作为中性粒细胞浸润和脂质过氧化的指标。α、β-香树脂醇以及地塞米松均显著抑制与牙周炎相关的TNF-α、牙龈MPO和TBARS的升高。α、β-香树脂醇在5 mg/kg时效果更显著。氯美昔布对所研究参数表现出不同影响。这些结果表明，α、β-香树脂醇可延缓大鼠牙周炎模型中的急性炎症，有必要进一步研究其预防慢性牙周炎相关骨质流失的疗效。
