Sánchez-Ramos Mariana, Encarnación-García José Guillermo, Marquina-Bahena Silvia, Sánchez-Carranza Jessica Nayelli, Bernabé-Antonio Antonio, Domínguez-Villegas Valeri, Cabañas-García Emmanuel, Cruz-Sosa Francisco
Departament of Biotechnology Autonomous Metropolitan University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1ª. Sección, Alcaldía Iztapalapa, México City 09310, Mexico.
Faculty of Chemical Science and Engineering, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, Mexico.
Pharmaceuticals (Basel). 2023 Oct 3;16(10):1400. doi: 10.3390/ph16101400.
(Kunth) R.M. King & H. Rob. belongs to the Asteraceae family and is a plant native to Mexico to which several biological properties are attributed. In this study, the cytotoxic effect of four extracts from the wild plants and two extracts from callus culture were evaluated against carcinogenic cell lines including prostate carcinoma, cervical cancer, hepatocellular carcinoma, hepatoma human, lung cancer, and cellular keratinocytes. The extracts were obtained with ethyl acetate and methanol using both leaves and stems or the callus. Only the ethyl acetate extract of the callus culture influenced the cervical cancer cell line (HeLa) with an IC of 94.79 ± 2.0 µg/mL. From the ethyl acetate callus extract, 2,3-dihydrobenzofuran was isolated and purified and also evaluated against cancer cells. The cytotoxic evaluation of this compound showed a significant effect against the HeLa cell line with an IC of 23.86 ± 2.5 µg/mL. Our results contribute to the development of biotechnological alternatives and extraction processes to produce compounds with possible potential against certain types of human cancer.
（孔茨）R.M. 金氏菊属植物属于菊科，是一种原产于墨西哥的植物，具有多种生物学特性。在本研究中，评估了野生植物的四种提取物和愈伤组织培养物的两种提取物对包括前列腺癌、宫颈癌、肝细胞癌、人肝癌、肺癌和细胞角质形成细胞在内的致癌细胞系的细胞毒性作用。使用叶子、茎或愈伤组织，用乙酸乙酯和甲醇提取这些提取物。只有愈伤组织培养物的乙酸乙酯提取物对宫颈癌细胞系（HeLa）有影响，其半数抑制浓度为94.79±2.0微克/毫升。从乙酸乙酯愈伤组织提取物中分离并纯化了2,3-二氢苯并呋喃，并对癌细胞进行了评估。该化合物的细胞毒性评估显示对HeLa细胞系有显著作用，其半数抑制浓度为23.86±2.5微克/毫升。我们的研究结果有助于开发生物技术替代方法和提取工艺，以生产对某些类型人类癌症可能具有潜在作用的化合物。
