Mathieu Bruno, Perrin Aurélie, Baldet Thierry, Delécolle Jean-Claude, Albina Emmanuel, Cêtre-Sossah Catherine
Entente InterDépartementale pour la démoustication, 165 Avenue Paul Rimbaud, 34184 Montpellier Cedex 4, France.
J Med Entomol. 2007 Nov;44(6):1019-25. doi: 10.1603/0022-2585(2007)44[1019:miowes]2.0.co;2.
In southern Europe, orbiviral diseases such as bluetongue (BT) have been assumed to have been largely transmitted by the classical Afro-Asian vector Culicoides imicola Kieffer (Diptera: Ceratopogonidae). Recent outbreaks have occurred in regions where C. imicola is normally absent, supporting the theory that other species belonging to the Obsoletus or Pulicaris complexes may play a role in BT virus transmission. Investigations of the ecology of the species within the former group are hampered by females of member species being extremely difficult to separate by classical morphology. To allow straightforward separation of these species in France, a multiplex polymerase chain reaction-based on internal transcribed spacer (ITS)-1 rDNA was developed to distinguish between Culicoides chiopterus Meigen, Culicoides dewulfi Goetghebuer, Culicoides montanus Shakirjanova, Culicoides obsoletus Meigen, and Culicoides scoticus Downes & Kettle. This tool will be useful in defining both the vector role and larval biotopes of these species in Europe.
在欧洲南部，诸如蓝舌病（BT）等环状病毒病一直被认为主要是由典型的亚非传播媒介库蠓（Culicoides imicola Kieffer，双翅目：蠓科）传播的。最近在通常没有C. imicola的地区爆发了疫情，这支持了一种理论，即属于Obsoletus或Pulicaris复合体的其他物种可能在蓝舌病病毒传播中发挥作用。由于前一组物种的雌性个体极难通过传统形态学进行区分，因此对其生态学的研究受到了阻碍。为了能在法国直接区分这些物种，开发了一种基于内转录间隔区（ITS）-1核糖体DNA的多重聚合酶链反应，以区分库蠓（Culicoides chiopterus Meigen）、德氏库蠓（Culicoides dewulfi Goetghebuer）、山地库蠓（Culicoides montanus Shakirjanova）、废弃库蠓（Culicoides obsoletus Meigen）和苏格兰库蠓（Culicoides scoticus Downes & Kettle）。该工具将有助于确定这些物种在欧洲的传播媒介作用和幼虫栖息地。
