Yoshida H, Honda T, Miwatani T
Research Institute for Microbial Diseases, Osaka University, Japan.
FEMS Microbiol Lett. 1991 Dec 1;68(3):249-53. doi: 10.1016/0378-1097(91)90364-g.
A hemolysin (designated Vm-rTDH) from Vibrio mimicus (AQ0915-E13) was purified by ammonium sulfate fractionation and successive column chromatography with DEAE-Sephadex A-25, hydroxyapatite, Mono Q, Superose 12 and Phenyl-Superose. The Mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. The isoelectric point of Vm-rTDH was approximately pH 4.9. The hemolytic activity of Vm-rTDH was stable upon heating at 100 degrees C for 10 min, similar to that of the thermostable direct hemolysin (Vp-TDH) of V. parahaemolyticus. Vm-rTDH also showed lytic activities similar to those of Vp-TDH. Immunological cross-reactivity between Vp-TDH and Vm-rTDH was demonstrated by the Ouchterlony double-diffusion test. Thus we conclude that V. mimicus produces a newly discovered type of hemolysin (Vm-rTDH) which is similar to Vp-TDH.
用硫酸铵分级沉淀法以及先后通过DEAE-葡聚糖A-25、羟基磷灰石、Mono Q、Superose 12和苯基-琼脂糖凝胶柱色谱法，从模仿弧菌（AQ0915-E13）中纯化出一种溶血素（命名为Vm-rTDH）。通过十二烷基硫酸钠平板凝胶电泳估计该亚基的相对分子质量约为22,000。Vm-rTDH的等电点约为pH 4.9。Vm-rTDH的溶血活性在100℃加热10分钟后仍保持稳定，这与副溶血性弧菌的耐热直接溶血素（Vp-TDH）相似。Vm-rTDH还表现出与Vp-TDH相似的溶解活性。通过双向免疫扩散试验证实了Vp-TDH和Vm-rTDH之间的免疫交叉反应性。因此，我们得出结论，模仿弧菌产生了一种新发现的与Vp-TDH相似的溶血素（Vm-rTDH）。
