Terai A, Shirai H, Yoshida O, Takeda Y, Nishibuchi M
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
FEMS Microbiol Lett. 1990 Sep 15;59(3):319-23. doi: 10.1016/0378-1097(90)90241-h.
The gene encoding a hemolysin similar to the thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus was previously cloned from the chromosome of Vibrio mimicus. The nucleotide sequence of the hemolysin gene was determined in this study. The gene proved to be a variant of the thermostable direct hemolysin gene (tdh gene) and was designated as Vm-tdh because the sequence divergences between the Vm-tdh gene and four tdh genes of V. parahaemolyticus were 2.1-3.0%, while the sequence divergences among the four tdh genes of V. parahaemolyticus ranged between 1.4 and 3.3%. Analysis of these five tdh genes revealed that they evolved from a common ancestor in discrete and understandable order by sporadic base substitutions.
先前已从模仿弧菌染色体中克隆出与副溶血性弧菌耐热直接溶血素（TDH）相似的溶血素编码基因。本研究确定了该溶血素基因的核苷酸序列。该基因被证明是耐热直接溶血素基因（tdh基因）的一个变体，并被命名为Vm-tdh，因为Vm-tdh基因与副溶血性弧菌的四个tdh基因之间的序列差异为2.1%-3.0%，而副溶血性弧菌的四个tdh基因之间的序列差异在1.4%至3.3%之间。对这五个tdh基因的分析表明，它们通过零星的碱基替换从一个共同祖先以离散且可理解的顺序进化而来。
