Placental stem cell markers in pre-eclampsia.
作者信息
Benian Ali, Uzun Hafize, Aydin Seval, Albayrak Mustafa, Uludağ Seyfettin, Madazli Riza
机构信息
Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
出版信息
Int J Gynaecol Obstet. 2008 Mar;100(3):228-33. doi: 10.1016/j.ijgo.2007.09.023. Epub 2007 Nov 28.
OBJECTIVE
To investigate the placental CD34, CD44, and leukemia inhibitory factor (LIF) levels in normotensive and pre-eclamptic women.
METHOD
The study population consisted of 21 women with pre-eclampsia. Twenty normotensive pregnant women served as controls and were matched to pre-eclamptic patients by gestational age at delivery. Decidual samples obtained from the central part of the placenta were stored at -70 degrees C until analysis. CD44 and LIF were quantified in homogenates by enzyme-linked immunosorbent assay (ELISA), while CD34 was quantified by flow cytometry.
RESULTS
The pre-eclamptic and normotensive groups were well matched. There were no significant differences in age, parity, weight, and gestational age at birth between the groups (P>0.05). The mean placental levels of CD34 (6.55+/-2.48 vs 3.16+/-1.23), CD44 (385.24+/-178.85 vs 157.75+/-31.73, and LIF (140+/-51.11 vs 96.25+/-31.62) were significantly higher in pre-eclamptic compared with normotensive women, respectively (P<0.05).
CONCLUSION
Higher levels of CD34, CD44, and LIF were found in the placentas of pre-eclamptic compared with normotensive women.
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