Cirilli Roberto, Ferretti Rosella, Gallinella Bruno, De Santis Emiliana, Zanitti Leo, La Torre Francesco
Istituto Superiore di Sanità, Dipartimento del Farmaco, Viale Regina Elena 299, 00161 Rome, Italy.
J Chromatogr A. 2008 Jan 4;1177(1):105-13. doi: 10.1016/j.chroma.2007.11.027. Epub 2007 Nov 17.
The chiral resolving ability of the amylose-based Chiralpak IA chiral stationary phase towards omeprazole and other proton pump inhibitors under reversed-phase conditions was investigated. Organic modifier-buffer demonstrated to be a valid alternative elution mode with respect to conventional polar organic and normal-phases. No evidence of deterioration of performance of the enantioselective column after several multimodal cycles of elution was observed. Mobile phase composition was systematically changed in order to modulate the enantiomer elution order of set of compounds studied. A very simple method based on on-line detection of optical rotational sign during enantioselective HPLC was developed to assign the absolute configuration and enantiomeric elution order.
研究了直链淀粉基手性固定相Chiralpak IA在反相条件下对奥美拉唑及其他质子泵抑制剂的手性拆分能力。与传统的极性有机相和正相相比，有机改性剂-缓冲液被证明是一种有效的替代洗脱模式。在多次多模式洗脱循环后，未观察到对映选择性柱性能下降的迹象。系统地改变流动相组成，以调节所研究化合物组的对映体洗脱顺序。开发了一种基于对映体选择性高效液相色谱法在线检测旋光符号的非常简单的方法，用于确定绝对构型和对映体洗脱顺序。
