在正相、极性有机和反相条件下，在手性pak IA上对卡瓦因进行对映体拆分。

Enantioseparation of kavain on Chiralpak IA under normal-phase, polar organic and reversed-phase conditions.

作者信息

Cirilli Roberto, Ferretti Rosella, Gallinella Bruno, Bilia Anna Rita, Vincieri Franco Francesco, La Torre Francesco

机构信息

Dipartimento del Farmaco, Istituto Superiore di Sanità, Rome, Italy.

出版信息

J Sep Sci. 2008 Jul;31(12):2206-10. doi: 10.1002/jssc.200800044.

DOI:10.1002/jssc.200800044

PMID:18563742

Abstract

First baseline HPLC enantioseparation of kavain is described. Complete enantiodiscrimination was achieved on the immobilised-type Chiralpak IA chiral stationary phase (CSP) using pure methanol and simple methanol-water and ethanol-water mixtures as eluents. A water-dependent enantioselectivity was clearly demonstrated. Performance of the Chiralpak IA CSP in polar organic and RP conditions was compared with that of five coated polysaccharide-derived CSPs used in normal-phase mode.

摘要

首次描述了卡瓦因的基线高效液相色谱对映体分离。使用纯甲醇以及简单的甲醇 - 水和乙醇 - 水混合物作为洗脱剂，在固定型手性派克IA手性固定相（CSP）上实现了完全的对映体鉴别。清楚地证明了对映选择性与水有关。将手性派克IA CSP在极性有机和反相条件下的性能与五种用于正相模式的涂覆多糖衍生CSP的性能进行了比较。