配体交换离子色谱法结合光度检测测定苹果汁中苹果酸对映体
Ligand-exchange ion chromatographic determination of malic acid enantiomers in apple juice with photometric detection.
作者信息
Yamamoto A, Matsunaga A, Mizukami E, Hayakawa K, Miyazaki M
机构信息
Toyama Institute of Health, Japan.
出版信息
J Chromatogr. 1991 Nov 1;585(2):315-7. doi: 10.1016/0021-9673(91)85093-u.
PMID:1804844
Abstract
An ion chromatographic separation with photometric detection using a chiral copper(II) complex as the eluent has been developed for the resolution of enantiomers of malic acid in commercially available apple juices. The results obtained by this method were in good agreement with those by an enzymatic method with separation by high-performance liquid chromatography.
摘要
已开发出一种离子色谱分离方法，该方法使用手性铜（II）配合物作为洗脱液，并通过光度检测来分离市售苹果汁中苹果酸的对映体。该方法得到的结果与通过高效液相色谱分离的酶法结果高度一致。
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