通过稀释计数估算病毒浓度：一种在袖珍计算器上编程的可靠计算方法。

Estimating viral concentrations from dilution counts: a reliable computation method programmed on a pocket calculator.

作者信息

Roussel P, Husson J

机构信息

IMEC v.z.w., Département MAP/ARS, Heverlee, Belgium.

出版信息

J Virol Methods. 1991 Sep-Oct;34(2):113-40. doi: 10.1016/0166-0934(91)90093-f.

DOI:10.1016/0166-0934(91)90093-f

PMID:1804848

Abstract

A program for a Hewlett-Packard 41C calculator is described, applying the maximum likelihood method for the estimation of the density of a suspension of infective particles when counts are available for a number of independent dilutions of the original suspension. An unbiased estimate for the variance of the density, a suitable confidence interval and its corresponding confidence level are also given by the program. Moreover, after testing for the presence of overcrowding and/or clumping, the selection of suitable dilutions is fully automated so as to avoid recourse to statistical tables.

摘要

本文描述了一个适用于惠普41C计算器的程序，当有原始悬浮液多次独立稀释后的计数结果时，该程序应用最大似然法来估计感染性颗粒悬浮液的密度。该程序还给出了密度方差的无偏估计、合适的置信区间及其相应的置信水平。此外，在测试是否存在过度拥挤和/或聚集现象之后，合适稀释度的选择完全自动化，从而无需查阅统计表。