Xu Jing-Liang, He Jian, Wang Zhi-Chun, Wang Kun, Li Wen-Jun, Tang Shu-Kun, Li Shun-Peng
Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2754-2757. doi: 10.1099/ijs.0.65095-0.
A Gram-positive, aerobic, non-motile, mesophilic strain, djl-6(T), able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from Jiangsu province, China. The taxonomic position of this isolate was analysed by using a polyphasic approach. Chemotaxonomic analysis including peptidoglycan type, diagnostic sugar composition, fatty acid profile, menaquinones, polar lipids and mycolic acids showed that the characteristics of strain djl-6(T) were in good agreement with those of the genus Rhodococcus. DNA-DNA hybridization showed that it had low genomic relatedness with Rhodococcus baikonurensis DSM 44587(T) (31.8 %), Rhodococcus erythropolis DSM 43066(T) (23.8 %) and Rhodococcus globerulus DSM 43954(T) (17.7 %), the three type strains to which strain djl-6(T) was most closely related based on 16S rRNA gene sequence analysis (99.78, 99.25 and 98.91 % similarity, respectively). Based on the phenotypic properties and DNA-DNA hybridization data, strain djl-6(T) (=CGMCC 1.6580(T) =KCTC 19205(T)) is proposed as the type strain of a novel Rhodococcus species, Rhodococcus qingshengii sp. nov.
从中国江苏省多菌灵污染的土壤样品中分离出一株革兰氏阳性、需氧、不运动的嗜温菌株djl-6(T)，该菌株能够降解多菌灵。采用多相分类法分析了该分离株的分类地位。包括肽聚糖类型、诊断性糖组成、脂肪酸谱、甲萘醌、极性脂质和分枝菌酸在内的化学分类分析表明，菌株djl-6(T)的特征与红球菌属的特征高度一致。DNA-DNA杂交显示，它与拜科努尔红球菌DSM 44587(T)（31.8%）、红平红球菌DSM 43066(T)（23.8%）和球形红球菌DSM 43954(T)（17.7%）的基因组相关性较低，这三个模式菌株是基于16S rRNA基因序列分析与菌株djl-6(T)关系最密切的菌株（相似性分别为99.78%、99.25%和98.91%）。基于表型特征和DNA-DNA杂交数据，菌株djl-6(T)（=CGMCC 1.6580(T)=KCTC 19205(T)）被提议作为一个新的红球菌属物种——清盛红球菌（Rhodococcus qingshengii sp. nov.）的模式菌株。
