Mederos Lilian, Valdivia José A, Valero-Guillén Pedro L
Laboratorio Nacional de Referencia e Investigaciones en Tuberculosis y Micobacterias, Centro Colaborador OPS/OMS, Instituto de Medicina Tropical Pedro Kourí (IPK), La Habana, Cuba.
Departamento de Genética y Microbiología, Facultad de Medicina y Odontología, Universidad de Murcia, Spain.
Microbiology (Reading). 2007 Dec;153(Pt 12):4159-4165. doi: 10.1099/mic.0.2007/011262-0.
Structural analysis of mycolic acids from Mycobacterium simiae (including some 'habana' strains) was carried out using (1)H-NMR and MS. Results indicated that this species presents a general pattern of alpha-, alpha'- and keto-mycolates. alpha-Mycolates were composed of a complex mixture of 82 to 89 carbon atoms (C82-C89), with the predominant molecular species containing two di-substituted cyclopropane rings. Among keto-mycolates (C84-C89), those containing one trans di-substituted cyclopropane ring were the most abundant. The alpha'-mycolates were monounsaturated (C64, C66). According to MS and (1)H-NMR data, the strains studied differed in fine structural details of alpha-mycolates and keto-mycolates. Notably, strain 'habana' TMC 5135 (belonging to the 'habana' group, and considered as highly immunogenic in tuberculosis and leprosy) presented a particular composition of alpha-mycolates, with a major component (C87) containing one cis plus one trans di-substituted cyclopropane ring, unlike the type strain of M. simiae and other strains of the 'habana' group (IPK-220 and IPK-337R), in which the major component (C84) contained two cis di-substituted cyclopropane rings. In spite of this finding, the 'habana' strains were closely related to each other and mainly differed from the type strain of M. simiae in some details of the fine structure of keto-mycolates. The present work indicated that within an identical general pattern of mycolic acids, there is a complex composition in M. simiae and structural variation among different strains, as reported for pathogenic species of the genus. Noteworthy was the particular composition of alpha-mycolates in strain 'habana' TMC 5135.
利用¹H-NMR和质谱对来自西氏分枝杆菌（包括一些“哈瓦那”菌株）的分枝菌酸进行了结构分析。结果表明，该菌种呈现出α-、α'-和酮型分枝菌酸的一般模式。α-分枝菌酸由82至89个碳原子（C82-C89）的复杂混合物组成，主要分子种类含有两个二取代环丙烷环。在酮型分枝菌酸（C84-C89）中，含有一个反式二取代环丙烷环的最为丰富。α'-分枝菌酸是单不饱和的（C64、C66）。根据质谱和¹H-NMR数据，所研究的菌株在α-分枝菌酸和酮型分枝菌酸的精细结构细节上存在差异。值得注意的是，“哈瓦那”菌株TMC 5135（属于“哈瓦那”组，在结核病和麻风病中被认为具有高度免疫原性）呈现出α-分枝菌酸的特殊组成，其主要成分（C87）含有一个顺式加一个反式二取代环丙烷环，这与西氏分枝杆菌的模式菌株以及“哈瓦那”组的其他菌株（IPK-220和IPK-337R）不同，在这些菌株中主要成分（C84）含有两个顺式二取代环丙烷环。尽管有这一发现，“哈瓦那”菌株彼此密切相关，并且主要在酮型分枝菌酸精细结构的一些细节上与西氏分枝杆菌的模式菌株不同。目前的研究表明，在分枝菌酸的相同一般模式内，西氏分枝杆菌存在复杂的组成，并且不同菌株之间存在结构变异，正如该属的致病菌种所报道的那样。值得注意的是“哈瓦那”菌株TMC 5135中α-分枝菌酸的特殊组成。
