Mederos Lilian M, Valdivia José A, Sempere Marco A, Valero-Guillén Pedro L
Instituto Pedro Kourí,La Habana, Cuba.
Hospital Costa del Sol, Marbella, Málaga, Spain.
Microbiology (Reading). 1998 May;144 ( Pt 5):1181-1188. doi: 10.1099/00221287-144-5-1181.
Fatty and mycolic acids and the pattern of glycolipids were studied in a collection of 34 strains of 'Mycobacterium habana' and in two strains of Mycobacterium simiae. Major glycolipids of these micro-organisms were assigned to the glycopeptidolipid (GPL) structural type, but both mycobacteria differed in the patterns obtained by TLC. The strains of 'M. habana' were separated into four groups (A-D), taking into account the presence or absence of several polar GPLs: group A contained GPL-I, GPL-II and GPL-III; group B contained GPL-I, GPL-II', GPL-II and GPL-III; group C contained GPL-II', GPL-II and GPL-III; group D did not contain any of these compounds. Fatty acids of both bacteria were similar, and ranged from 14 to 26 carbon atoms, hexadecanoic, octadecenoic and tuberculostearic acids being predominant. Mycolic acids were also similar by TLC and HPLC, and consisted of alpha-, alpha'- and ketomycolates. Partial structural analysis by MS carried out in strains 'M. habana' TMC 5135 and M. simiae ATCC 25275T revealed that alpha- and ketomycolates ranged, in general, from 79 to 87 carbon atoms, and alpha'-mycolates from 58 to 67 carbon atoms. The alpha- and ketomycolates belonged to several structural series, and minor variations were found between the two strain examined. The data obtained justified the synonymy between 'M. habana' and M. simiae but indicated, in turn, that the former can be distinguished on the basis of GPL analysis. Most strains of 'M. habana' can be defined by the presence of GPL-II and GPL-III, a finding that could be useful in the quality control of potential vaccine strains.
对34株“哈瓦那分枝杆菌”菌株和2株西氏分枝杆菌菌株的脂肪酸、霉菌酸和糖脂模式进行了研究。这些微生物的主要糖脂被归为糖肽脂（GPL）结构类型，但两种分枝杆菌在薄层色谱（TLC）得到的模式上有所不同。考虑到几种极性GPL的存在与否，“哈瓦那分枝杆菌”菌株被分为四组（A - D）：A组含有GPL - I、GPL - II和GPL - III；B组含有GPL - I、GPL - II'、GPL - II和GPL - III；C组含有GPL - II'、GPL - II和GPL - III；D组不含这些化合物中的任何一种。两种细菌的脂肪酸相似，碳原子数范围为14至26个，十六烷酸、十八碳烯酸和结核硬脂酸占主导。通过TLC和高效液相色谱（HPLC）分析，霉菌酸也相似，由α - 、α' - 和酮型霉菌酸组成。对“哈瓦那分枝杆菌”TMC 5135菌株和西氏分枝杆菌ATCC 25275T菌株进行的质谱（MS）部分结构分析表明，α - 和酮型霉菌酸一般含有79至87个碳原子，α' - 霉菌酸含有58至67个碳原子。α - 和酮型霉菌酸属于几个结构系列，在所检测的两个菌株之间发现了微小差异。所获得的数据证明了“哈瓦那分枝杆菌”和西氏分枝杆菌之间的同义关系，但反过来也表明前者可以通过GPL分析加以区分。大多数“哈瓦那分枝杆菌”菌株可以通过GPL - II和GPL - III的存在来定义，这一发现可能对潜在疫苗菌株的质量控制有用。
