Encheva V, Wait R, Begum S, Gharbia S E, Shah H N
Molecular Identification Services Unit, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK.
Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College, ARC Building, 1 Aspenlea Road, London W6 8LH, UK.
Microbiology (Reading). 2007 Dec;153(Pt 12):4183-4193. doi: 10.1099/mic.0.2007/010140-0.
Salmonella enterica is one of the most extensively studied bacterial species in terms of physiology, genetics, cell culture and development. As a very diverse group, the serovars of S. enterica display a spectrum of host specificities ranging from a broad host range to strictly host-adapted variants. This study utilized a classic proteomic approach combining 2D gel electrophoresis and mass spectrometry for the comparative analysis of the proteomes of serovars Typhimurium, Enteritidis, Choleraesuis, Pullorum and Dublin. The comparative analysis revealed species-specific protein factors with no significant change in expression amongst all isolates, as well as proteins with fluctuating expression levels between serovars and strains. Examples include an isoform of SodA specific for serovar Typhimurium, the third isoform of the lysine arginine ornithine (LAO)-binding amino acid transporter specific for serovar Pullorum, and the enzyme GabD found to be unique to serovar Choleraesuis. Overall the study demonstrated the importance of using multiple isolates when characterizing the expression patterns of bacteria in order to account for the intrinsic diversity of a bacterial population and revealed several factors with potential roles in host adaptation and pathogenicity of the serovars of S. enterica.
就生理学、遗传学、细胞培养和发育而言，肠炎沙门氏菌是研究最为广泛的细菌物种之一。作为一个非常多样化的群体，肠炎沙门氏菌的血清型表现出一系列宿主特异性，从广泛的宿主范围到严格适应宿主的变体。本研究采用经典蛋白质组学方法，结合二维凝胶电泳和质谱，对鼠伤寒血清型、肠炎血清型、猪霍乱血清型、鸡白痢血清型和都柏林血清型的蛋白质组进行比较分析。比较分析揭示了所有分离株中表达无显著变化的物种特异性蛋白质因子，以及血清型和菌株之间表达水平波动的蛋白质。例如，鼠伤寒血清型特有的SodA同工型、鸡白痢血清型特有的赖氨酸精氨酸鸟氨酸（LAO）结合氨基酸转运蛋白的第三种同工型，以及猪霍乱血清型特有的酶GabD。总体而言，该研究证明了在表征细菌表达模式时使用多个分离株的重要性，以便考虑细菌群体的内在多样性，并揭示了几个在肠炎沙门氏菌血清型的宿主适应性和致病性中可能起作用的因素。
