Chu C, Hong S F, Tsai C, Lin W S, Liu T P, Ou J T
Department of Microbiology and Immunology, Chang Gung University College of Medicine, Kweishan, Taoyuan, Taiwan.
Infect Immun. 1999 May;67(5):2611-4. doi: 10.1128/IAI.67.5.2611-2614.1999.
Using fragment profiling, PCR, and Southern hybridization, we found that Salmonella enterica serovar Choleraesuis harbored virulence plasmids of various sizes, whereas serovars Typhimurium, Enteritidis, and Dublin carried a plasmid of a unique size. Also, the virulence plasmid of Typhimurium contained genes in the same order detected in the other three plasmids, all of which contained deletions.
通过片段分析、聚合酶链反应(PCR)和Southern杂交,我们发现猪霍乱沙门氏菌携带各种大小的毒力质粒,而鼠伤寒沙门氏菌、肠炎沙门氏菌和都柏林沙门氏菌携带单一大小的质粒。此外,鼠伤寒沙门氏菌的毒力质粒所含基因的排列顺序与其他三种质粒中检测到的相同,所有这些质粒都有缺失。